thr77 login Options
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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which leads to chromosome instability, and so may well lead to tumorigenesis. This protein was located to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential part in selling cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants have been noted but thorough nucleotide sequence info is just not yet readily available.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide commonly incorporates the epitope recognized through the antibody.
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This manual outlines even more probable optimization of cellular and immunoassay parameters to make certain the absolute best effects are...
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This guidebook outlines even more attainable optimization of cellular and immunoassay parameters to be sure the absolute best final results are...
Antibodies certain to the blocking peptide no longer bind to the epitope on the concentrate on protein. By evaluating the staining from your blocked antibody versus the antibody by itself, you can see which staining is certain.
Target Information and facts Cyclin E belongs into the remarkably conserved cyclin household, whose members are characterised by a remarkable periodicity in protein abundance in the cell cycle. Cyclins functionality as regulators of CDK kinases. Diverse cyclins show unique expression and degradation styles which contribute to your temporal coordination of each mitotic celebration. Cyclin E forms a fancy with and capabilities as being a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S phase boundary and is also degraded as cells development as a result of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...
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